By Ray H. Gavin
Ray H. Gavin brings jointly a global panel of skilled researchers to element the with ease reproducible equipment that make the most of biochemistry, immunology, genetics, microscopy, and photograph research for investigating cytoskeleton constitution and serve as. each one protocol includes confirmed step by step directions that permit either the amateur and the skilled researcher to accomplish winning experimental effects. The protocols make the most of diversified version platforms in numerous organisms, together with Saccharomyces, Micrasterias, Tetrahymena, Drosophila, Spisula, and Xenopus. Microscopy functions comprise digital-video microscopy and computer-assisted platforms for the assessment of mobile motility and morphology. state of the art and hugely sensible, Cytoskeleton equipment and Protocols makes to be had a various choice of strong experimental structures and instruments for effectively learning cytoskeleton constitution and serve as.
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Extra info for Cytoskeleton Methods and Protocols (Methods in Molecular Biology Vol 161)
46, 606–611. 22. Lincoln, L. , Gibson, T. , Forney, J. , and Asai, D. J. (1998) The characterization and targeted disruption of the dynein beta heavy chain in Tetrahymena thermophila. Mol. Biol. Cell 9, 155a. Video-Enhanced Microscopy II MICROSCOPY APPLICATIONS 29 30 Langford Video-Enhanced Microscopy 31 4 Video-Enhanced Microscopy for Analysis of Cytoskeleton Structure and Function George M. Langford 1. 1. Cytoskeletal Dynamics and AVEC-DIC Microscopy The cytoskeleton is a dynamic network of filaments in the cytoplasm of cells and functions as the roadways for vesicular transport.
The extent of ligation is sensitive to the proportion of insert and vector; therefore, it is a good idea to set up two or three ligations varying the insert-to-vector ratio. 5. This protocol is based on a procedure described by Meng-Chao Yao’s laboratory (19). 6. These methods are based on standard protocols (8). 7. A Southern blot of EcoRI-digested genomic DNA was probed with the mixture of PCR-amplified fragments. The result is shown in the far left lane of Fig. 3. Approximately 13–14 hybridizing bands were seen in this blot.
47 Late model Macintosh computer (Apple Computer Inc). Adobe Premiere software (Adobe Systems Inc, San Jose, CA). 3D-DIAS Software (Solltech Inc). Crystal Eyes 3D display system (Stereographics, San Raphael, CA). 3. 3D-DIAS (Confocal) Experiment 1. Dvorak-Stotler perfusion chamber (Lucas-Highland). 2. Zeiss Axiovert S100TV inverted microscope (Carl Zeiss). 3. Noran Oz Laser Scanning Confocal Microscope (LSCM) (Noran Corporation, Middleton, WI). 4. 100× Plan-Neofluar oil immersion objective (Carl Zeiss).